منابع مشابه
Multiphoton fluorescence microscopy.
Multiphoton fluorescence microscopy has now become a relatively common tool among biophysicists and biologists. The intrinsic sectioning achievable by multiphoton excitation provides a simple means to excite a small volume inside cells and tissues. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal c...
متن کاملMultiphoton Fluorescence Microscopy
The human body is a constant flux of thousands of chemical/biological interactions and processes connecting molecules, cells, organs, and fluids, throughout the brain, body, and nervous system. Up until recently it was thought that all these interactions operated in a linear sequence, passing on information much like a runner passing the baton to the next runner. However, the latest findings in...
متن کاملOptimizing 3D multiphoton fluorescence microscopy.
We present a new optimization concept for 3D multiphoton fluorescence microscopy by finding the optimal excitation beam giving rise to the smallest possible light-emitting volume or the highest possible signal to noise ratio (SNR).
متن کاملMaximizing fluorescence collection efficiency in multiphoton microscopy
Understanding fluorescence propagation through a multiphoton microscope is of critical importance in designing high performance systems capable of deep tissue imaging. Optical models of a scattering tissue sample and the Olympus 20X 0.95NA microscope objective were used to simulate fluorescence propagation as a function of imaging depth for physiologically relevant scattering parameters. The sp...
متن کاملCharacteristics of subgingival calculus detection by multiphoton fluorescence microscopy.
Subgingival calculus has been recognized as a major cause of periodontitis, which is one of the main chronic infectious diseases of oral cavities and a principal cause of tooth loss in humans. Bacteria deposited in subgingival calculus or plaque cause gingival inflammation, function deterioration, and then periodontitis. However, subgingival calculus within the periodontal pocket is a complicat...
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ژورنال
عنوان ژورنال: Methods
سال: 2001
ISSN: 1046-2023
DOI: 10.1006/meth.2001.1219